The structure, characterization and dual-activity of exopolysaccharide produced by Bacillus enclensis AP-4 from deep-sea sediments

In recent years, the exopolysaccharide (EPS) produced by deep-sea bacteria has attracted interest of various researchers. present study, we have explored properties and structure a novel (called BPS) Bacillus enclensis AP-4 from sediments. The maximum yield BPS was 4.23 ± 0.17 g L ?1 in 2216E modified medium. 1 H NMR studies purified displayed ? ?-configuration sugar residues, including mannose, glucosamine, glucose, galactose, xylose molar ratio 1.00: 0.09: 0.04: 0.07. showed molecular weight 23,434 Da abundant hydroxyl amino residues. addition, exhibited rod-like with rough surface dominated C, N, O elements. demonstrated remarkable thermal stability, high degradation temperature, excellent emulsification capacity compared to most reported exopolysaccharides. Moreover, better quenching activities against four radicals, which provided favorable protection for strain. Finally, freezing experiment investigated cryoprotective effect on E. coli S. aureus . effectively improved cell survival maintained activity Na + /K -ATPase, facilitates culture preservation. To best our knowledge, work is first report suggesting that marine dual-activity. This presents foundation analysis exopolysaccharides bacteria.